The mechanism of action of Escherichia coli heat-stable toxin.
نویسنده
چکیده
Holmgren, J. (1981) Nature (London) 292, 413417 Hughes, J . M., Murad, F., Chang, B. & Guerrant, R. L. (1978) Nature (London) 271, 755-756 Hughes, S., Warhurst, G., Turnberg, L. A,, Higgs, N. B., Giugliano, L. G. & Drasar, B. S. (1983) Gut 24 (2), 94-98 James, C., Dibley, M., Burke, V., Robinson, J. & Gracey, M. (1982) Clin. Expt. Immunol. 47, 3 4 4 2 Leppla, S. H. (1982) Proc. Natl Acad. Sci. U.S.A. 79, 3162-3166 Moss, J. & Vaughan, M. (1980) in Secretory Diarrhea (Field, M., Fordtran, J . S. & Schultz, S. G., eds.), pp. 107-126, American Physiological Society, Bethesda 11787-11792
منابع مشابه
Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR
The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofEscherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of Shahrekord. Polymerase chain reaction assays were used to detect t...
متن کاملConstruction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
متن کاملMolecular characterization and antibiotic resistance of enterotoxigenic and entero-aggregative Escherichia coli isolated from raw milk and unpasteurized cheeses
The aim of this study was to determine the occurrence of enterotoxigenic and enteroaggregative Escherichia coli strains and antibiotic resistance of the isolates in raw milk and unpasteurized cheese. Out of 200 samples of raw milk and 50 samples of unpasteurized cheeses, 96 and 24 strains of E. coli were isolated, respectively. Polymerase chain reaction (PCR) was used to detec...
متن کاملESCHERICHIA COLI HEAT-LABILE TOXIN B SUBUNIT: CONSTRUCTION AND EVALUATION OF PLASMIDS PROVIDING CONTROLLED HIGH LEVEL PRODUCTION OF THE PROTEIN
With the plasmid DNA from a clinical isolate of enterotoxigenic Escherichia coli (ETEC) H 10407 as template, PCR-mediated cloning of the sequence encoding the heat-labile toxin B subunit (L T -B) has been carried out. Then this sequence was recloned into the pTrc 99A and pET23a expression vectors to give the pJasmids pTRCLTB and pETLTB, respectively. After induction, the former plasmid provides...
متن کاملDoes enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin A subunits?
Although most enteropathogenic Escherichia coli strains do not produce recognized enterotoxins, we wished to examine whether they produce any factors like heat-stable enterotoxin b or cholera toxin active subunits that might be missed by conventional assay methods. E. coli strains E851 (O142) and E2348 (O127) that had caused diarrhea in volunteers were negative for heat-labile enterotoxin and h...
متن کاملConstruction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
Hepatitis B surface antigen is the first genetically engineered vaccine licensed for human use. Various strategies have been proposed to obtain a vaccine that would bypass the need for injection. In this study, a non-toxic portion of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface antigen. T...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Biochemical Society transactions
دوره 12 2 شماره
صفحات -
تاریخ انتشار 1984